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RNA-seq analysis and differential expression guide v1.0.0

Use this resource to follow an RNA-seq analysis from quality control to differentially expressed genes

New

  • 197 downloads
  • 124 likes
  • 41 saves

Free

Install with the CLI

In a terminal, from the folder you want:

npx --yes palatekit@latest install rna-seq-differential-expression-guide

First time: connect the CLI with

npx --yes palatekit@latest login

The prompt asks the assistant to explain preprocessing and quality control, normalization methods and statistical approaches such as DESeq2 or edgeR. It ends with visualization tips, such as heatmaps or volcano plots, and common pitfalls. You get a reproducible analysis plan.

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Updated November 2, 2026

Card for the “RNA-seq analysis and differential expression guide” prompt

Details

Generates with
Your agent's model
Licence
CC0-1.0

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What you get

  • The full prompt, ready to paste
  • Steps, normalization, statistics and visualization
  • Variables to replace: ${dataQuality:high}, ${normalizationMethod:DESeq2}, ${visualizationTools:heatmap}

Good fit for

  • Planning an expression analysis for a research project
  • Learning the steps of an RNA-seq analysis before building a pipeline

Not for

    Tags

    • rna-seq
    • bioinformatics
    • expression
    • genomics
    • analysis

    About

    You say

    “Compare DESeq2 and edgeR for a design with three replicates per condition.”

    Card for the “RNA-seq analysis and differential expression guide” prompt

    Prompt published by rmfsantos@uefs.br under the CC0-1.0 license, delivered in its original version.

    Show more

    How to use it

    1. Paste the prompt into your assistant or agent
    2. Ask it to follow an RNA-seq analysis from quality control to differentially expressed genes
    3. Review and adjust the result

    Content preview

    Files

    • LICENSE0.6 KB
    • NOTICE-PALATEKIT.md0.5 KB
    • rna-seq-differential-expression-guide.md0.8 KB

    Origin and licence

    Listed unmodified

    Author
    rmfsantos@uefs.br
    Source
    github.com/f/prompts.chat/blob/cecef6fc73c2c389770695a56252d331981727de/prompts.csv
    Licence
    CC0-1.0

    Notice

    « RNA-Seq Analysis and Differential Gene Expression » (rmfsantos@uefs.br, CC0-1.0) livré dans sa version d'origine : fiche en français et en anglais par PalateKit.

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